Journal:

Article Title: Cloning, Expression, and Characterization of the katG Gene, Encoding Catalase-Peroxidase, from the Polycyclic Aromatic Hydrocarbon-Degrading Bacterium Mycobacterium sp. Strain PYR-1

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Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Pyrene was obtained from Aldrich Chemical Company (Milwaukee, Wis.). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Description Reference or source Strains Mycobacterium sp. strain PYR-1 Mineralizes high-molecular-weight PAHs such as fluoranthene, pyrene, and phenanthrene 8 Escherichia coli XL1-Blue MRF′ lac [F′ proAB lacI q ZΔM15 Tn 10 (Tet r )] Stratagene Escherichia coli XLOLR lac [F′ proAB lacI q ZΔM15 Tn 10 (Tet r )] Su − (nonsuppressing) λ r Stratagene Escherichia coli TOP10 One Shot competent cells, F′ mcrA Δ( mmr-hsdRMS-mcrBC ) Φ80 lacZ ΔM15 Δ( ara-leu ) 7697 Invitrogen Plasmid or vector ExAssist helper phage For in vivo excision of the pBK-CMV phagemid from the ZAP Express vector with E. coli XLOLR Stratagene Zap Express vector Lambda vector, prokaryotic and eukaryotic expression, in vivo excision of the pBK-CMV vector Stratagene pBK-CMV vector Neo r Kan r , ColE1 origin, lacZ , cytomegalovirus promoter Stratagene Myco-pBK-CMV phagemid pBK-CMV phagemid with 1.8-kb insert from Mycobacterium sp. strain PYR-1 This study pBAD/Thio-TOPO vector Amp r pMB1 origin, six-His-Thioredoxin open reading frame, arabinose induced Invitrogen katG -pBAD/Thio plasmid pBAD/ThioFusion with the katG gene of Mycobacterium strain PYR-1 This study Open in a separate window Bacterial strains and plasmids used in this study PAH induction of the Mycobacterium sp. cultures and two-dimensional (2D) gel electrophoretic analysis of recovered proteins.

Techniques: Plasmid Preparation, In Vivo, Expressing